FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880261
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACT3554.7036254E-516.3357681
TTTGATC7753.0136844E-510.2345042
CTTTCGA6650.0091163788.7205981
CTTAAAA10901.3957586E-58.6455931
CTTCAAC10407.584774E-58.3642271
CTCATTG8100.00497329248.05444051
CTTTAAT12003.9776432E-47.24899671
CTTTGTA11750.00253078236.7862951
CTTTGAT15651.7151429E-46.4847261
ATCAAAT14850.00442398675.85777471
CAAAAGA17000.00323070685.5433511
CTGCTTT19600.0030267185.1778551
Produced by FastQC (version 0.11.5)