FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1056421
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT4650.023.8229059
ACACGTC5600.019.7815178
CACACGT6200.018.9838757
AGCACAC8450.015.5677045
AGAGCAC7605.456968E-1215.485383
GCACACG8252.0008883E-1114.2666716
GAGCACA8352.3646862E-1114.096484
AGAGTTA3500.001105930913.8470635
GAAGAGC12702.2955646E-99.8347611
GTTTCGT7855.662562E-48.8394521
AAGAGCA14001.1996963E-78.4087372
CGGTTTG11100.00225583236.8611585
AAAAAAA20750.05.672666560-64
GTTCCGG17550.0078563925.1399771
Produced by FastQC (version 0.11.5)