FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1823520
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAAC12355.9609236E-57.7005651
ACTTACA10000.0041668447.22042236
CTCATTA12405.225021E-47.0795511
CTTGGAT10700.0070777376.836951
CTCCTGA17251.0759093E-56.78542571
CTTGCTT18905.855176E-66.5801121
CCCAGCT13900.00190949046.31557131
CATCGAT14150.00233303736.2039881
TTAAAAC20651.7939291E-45.59344242
CTTTACT17050.00300754335.57782841
CTTTCAA31601.2871715E-75.55610371
CTCATTT18950.001771355.4046181
CTTTAAA27501.069598E-55.320391
CAACAAT23801.9795084E-45.22538331
ATTCATT27255.192492E-55.1007411