FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494489
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTGA1952.712556E-422.1836091
ACTTCTA4453.6298687E-412.8445625
TCGAGGA3900.001966576312.82657052
CTCCATT5650.002485114410.2083871
CTTTAAA8659.187655E-610.0018591
CTTTTAA8054.9561117E-48.9561161
CTTGAAT6500.0078969718.8734451
CTTCTGC9300.0020716567.75233751
CTTTGAT9450.00242412847.6292841
TTTTAAA13452.271676E-46.9071392
CTTCTTT18903.313525E-45.7219631