FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035157
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC4300.026.8454638
CACACGT4200.025.766857
CACGTCT4505.456968E-1222.4457879
AGCACAC5801.0913936E-1118.6587545
GCACACG5802.2191671E-1017.4148376
GAGCACA6057.185008E-915.519194
AGAGCAC6401.5093974E-814.6704853
GAAGAGC10659.4499046E-79.5780521
AAGAGCA13151.8247945E-57.68923952
AATCCAG11502.5756352E-47.5284015
CCTCCTA4551.2377545E-45.392259630-34
ACTCCAG6102.8085688E-65.20405915-19
Produced by FastQC (version 0.11.5)