FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999721
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT4150.043.362517
ACACGTC5500.032.7189878
CACGTCT5950.030.2444429
AGAGCAC6400.028.1235053
AGCACAC6450.026.7852545
GCACACG7850.023.8410976
GAGCACA7700.023.3753814
AAGAGCA12700.014.1809882
GAAGAGC14750.011.767021
CAATTAA4750.008123307510.6099623
TCAATTA6150.005051569.3709812
GTTTGTC9502.6917493E-48.3736811
CTCCAGT6350.07.935395215-19
AGTCACG4901.2732926E-117.65810920-24
ACTCCAG5205.2750693E-117.19853715-19
CAGTCAC5051.9463187E-107.144847420-24
CGAATGA6505.456968E-126.659058630-34
GTCACGA7450.06.58668620-24
ACGTCTG6659.094947E-126.494660410-14
CTCGTAT6801.4551915E-116.368724335-39
Produced by FastQC (version 0.11.5)