FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1221132
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAATC6303.490038E-511.6480481
CTCCAGA12155.0893505E-89.6635651
ATACACG6200.0053848199.2988688
CACAGAG7350.00184950698.9856371
CTCCGAT10557.778661E-58.3468481
CTTATTT13352.0371845E-68.2452471
GTTCAAT9903.5885372E-48.1536341
CAACAAT14709.36805E-77.98723271
CCCAAAG8550.00724224567.72449451
CCTCAAA13451.9943762E-57.63834761
CCACAAA11903.1417204E-47.3999361
ATAAGAT12153.9896424E-47.2476741
CTTCATA15952.5223391E-56.90119461
CTTGTTT15058.790103E-56.82629781
CACAAAG13200.00102742396.6711551
CTTTGAT16654.5878245E-56.61105441
TCAGATT12200.00398941246.4985732
CAATTTT14454.9387885E-46.4834674
ATTCAAT13600.00144056996.4749441
CTCAAAA13950.00191848566.31249051
Produced by FastQC (version 0.11.5)