FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729163
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC3350.00926865213.0867471
GTTCAAC6100.00419933869.5826451
AAACTTA6200.00532275769.3110525
CCCATGC7400.0020435138.8866091
CTTGTTT8759.959621E-48.3505911
CTTCAAA14004.2547344E-67.8286791
CCTTGAT8700.0087837717.5587251
CCTCTTC9750.00289224417.494121
AAGTTTT10750.0084366676.7126193
CTCTTTT13000.00669489946.18264961
Produced by FastQC (version 0.11.5)