FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865277
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAAT3000.00469640414.50229551
TAACCGT5450.001803700310.6112827
CTTCGAA6557.1894773E-49.9634081
CCCAAAT6500.00754386658.924491
CTCCCAG6700.0096686588.6580871
CAACAAG9251.9591278E-48.6229861
CTTGTAT8150.00524206278.0074021
CTATAAA9250.00186041577.83907841
CTTGGAA10356.566851E-47.70653441
CTCCATT10000.00399279737.25114771
CCTTGAT11100.0013831737.1858221
CAATTTT11100.00143152857.16256864
CTTCAAA15251.5879832E-57.13227651
CTTAAAA11200.00152104747.1216631
CATTCTT14858.98917E-56.8139774
CTTTATT14959.406716E-56.79037241
CTTCATT14554.977555E-46.47868871
CAAAGAT14450.00325230956.0217141
CTTTTAT18050.00106825625.62415931
Produced by FastQC (version 0.11.5)