FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497206
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGAGG2003.2686407E-421.58028
TAGCACC2000.00829539517.9762679
CTTCTAA4300.003891825211.7101878
CCTTAAA4450.004929648711.3451371
CTTCCAT8951.206623E-610.4759161
CTTCTCT7351.9830288E-49.8126061
CTTTTAA8701.0646636E-49.1189561
CTTCAAT9904.310062E-48.0136281
CTTATTT8700.0098547817.4609641
TAAGCGT2150.00105762497.371070495-99
CTCCTTT10400.0061486266.93487071
GTTCTTT12708.038199E-46.81473881
Produced by FastQC (version 0.11.5)