FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2257088
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAAG5852.3634471E-411.2410131
CTTCGAT20357.4942363E-107.899091
CTCCGTT18607.815834E-87.46379041
CTTAGAA9850.00320270057.41792631
CCTTAAG10500.0059667336.95872161
CTCAAAT21301.1421071E-76.8607111
CTCGATT12050.0030400546.66997771
CCCTGAT19951.9670497E-66.59247351
CTTCAAT35500.06.58628271
CTATTGT16956.249116E-56.46606871
CTCAGAA16102.2415584E-46.3536151
CTCTCAT17651.0919708E-46.20962431
CCCCTTT16603.328021E-46.16224151
CCCAAAT14400.00287871346.08888151
CTTCGAG16150.00160052875.88152031
CTCCGCT17657.3081977E-45.79564951
CTTTAAT31602.1816959E-85.78058341
CCCATTT23302.4382756E-55.6446281
CTCACCG17050.00305216275.57105881
CTCATAT23803.422222E-55.5260441
Produced by FastQC (version 0.11.5)