FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468495
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT1500.001551208424.00879
GTTGGTA2450.001270259517.6390468
CTCTTTA4500.004681985411.42353251
CTTCAGA5200.001066996611.2979971
CAAATAA6050.0044122739.5241123
TTTCCTT10350.0059432126.9590438
CTTTCTT11700.00210507946.9043321
ATTTCTT13300.0098176885.9570467
Produced by FastQC (version 0.11.5)