FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891812
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCATGC6455.8157044E-410.1959211
CTACAAC7400.00204366688.8869861
CCTGCAT14651.04762876E-78.4792011
CTTTAAA14708.49772E-67.4562011
CTTTGAA16809.3432755E-77.3940661
CATTGTT11750.00232694116.8406681
CTTTCAA14755.352054E-46.4401361
TTTTGTA13650.00180027356.34778937
CTTTGAT13900.00193133286.3082681
ATTTTAC12600.00547655356.3037076
GAACTGC12900.0069931076.15710935
TTCAATG14200.00280528826.1019242
ATTTTCC16754.3408226E-46.0351376
CTTCATT14550.00322406236.02645541
CTTTTAT16050.00148157985.91850471
ACCAAGT3700.0091423675.85454853
CTTCAAA18752.487499E-45.84566161
AATTTTC16250.00198048265.77648835
ATCAAAT16050.0095621225.4632351
CTTTCCG17400.00387019045.459311
Produced by FastQC (version 0.11.5)