FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1893759
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAGG9552.893509E-59.0818281
CTCTGAT15904.6518835E-77.72764541
CTCTAAA10350.00576590146.9832091
GACTAAG3202.3888806E-56.9297047130-134
CTCAAAT16707.964396E-66.92466741
AAGTACG10750.0086378426.6974456
CTCAGAA13150.00117017126.5955481
CTCGTTT14250.00289111586.08641771
CCCTGAA17658.392422E-45.73295741
CTTGAAT19103.7183534E-45.6761421
ATCTATA17050.00347054595.51079651
CAATAAT34102.492925E-85.51079651
CTTCGGA17550.0048800175.3537931
CCCTGAT20308.445076E-45.34060671
Produced by FastQC (version 0.11.5)