FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960015
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC4350.037.2952358
CACACGT4400.036.8714267
GAGCACA5000.033.8575864
GCACACG5600.031.4895736
AGAGCAC5700.028.4621543
AGCACAC6050.026.8155825
CACGTCT6500.023.873949
GAAGAGC11400.016.1732231
AAGAGCA13700.012.8797012
CCCAATT4600.00716334610.7911641
CAACCTT7450.00285144228.5667161
CGAATGA7750.0040735158.23511
ATCGAAT8400.0083928377.5978611
AGTCACG4851.6370905E-117.58267520-24
CTCGTAT5403.6379788E-127.325899635-39
CAGTCAC5452.419256E-106.747884820-24
TGCATCT11650.00308315086.6601123
GTCACGT5204.2382453E-96.528279320-24
ATTTCCC13700.00244045476.17839246
TGTTTTA14959.653362E-46.13362074
Produced by FastQC (version 0.11.5)