FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3199201
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAT24452.447432E-76.2210741
CCCCGTT19809.352183E-55.85301641
CTCAATT17950.00101275075.6492211
GTTCCCT18100.00112556855.60240361
CCTCAAC22007.048516E-55.5969471
CAACTTT33304.7046524E-75.2202581
GTTTAAT19650.00316572075.16048431
CCTCAAT27155.7667065E-55.06884151
CTCCCAT21450.0017110485.06511071
GTCCCAG18600.0093978675.06238751
Produced by FastQC (version 0.11.5)