FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347281
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAACT4400.00413818811.6167131
CTCGGAT5950.00344041439.8177261
CTTGAAT14156.0628736E-88.7726411
CATGACT6700.0091375938.7187271
CACCCAT8457.099849E-48.6413411
CTGGAAT15051.6380545E-78.2480321
CCCAAAT8300.00580409177.9177591
CTGGCAT9450.0021455397.7269141
AGTATCA11703.0980652E-47.40911537
CTCCGAT12104.0292967E-47.2415871
CTTTGGA13551.9179525E-47.0055451
CTTAAAA12606.4044003E-46.95422271
CTACAAT12757.329731E-46.87240841
CTTTGAT20102.2439326E-66.53904531
CCAGCAT18002.0689391E-56.4906081
CCTCAAA13600.00152399326.44288251
CTCTTCT14805.631389E-46.41386031
CACCAAT12550.0046798016.4001011
CATCCAT16102.257812E-46.3495081
CCCGCAT13450.0096009425.9718421
Produced by FastQC (version 0.11.5)