FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635003
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAATC4453.195801E-413.040751
CACAAAG6956.176451E-712.5247481
TCAAAAC7301.7893521E-49.9141682
CTTCGTT7351.8731033E-49.8692741
CTTAAAA8901.2003253E-59.7805621
CTCAAAA8608.8246656E-59.2782651
CTTATAA7750.00331659158.4239031
CAACAAG12951.4003988E-57.8420721
CAAAACA10150.00470895747.1287033
CTTTTAT11450.00191119496.9688281
CTTTTTA12757.88263E-46.82721571
ATCAAGG11900.00286810616.70530131
CTTCTTG15700.0081204975.54439451
Produced by FastQC (version 0.11.5)