FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1355533
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCTT5009.5529034E-411.4512381
CTCCAAG8150.0058991377.9034621
CTTACGG11402.556527E-47.5337091
CTCATTT13352.5186844E-57.5054931
CTTCAAC15352.6200978E-67.46008971
CTCAAAA14458.956555E-67.4294361
AAACGAC9750.00358075797.33322575
CATGAAT13702.8064713E-46.79133651
CTAAAAT12950.00109867836.63199141
CTTAAAA18153.1348838E-56.30922171
CCCAAAA12600.00600978646.2481951
CCCAGAA14150.0029804816.06956051
GTTCAAA16250.00220327995.72561841
CCTTTAT16600.00283815395.60489751
ACGATAT15550.0085848565.5176043
CGATATT17150.00422215555.41974544
CTTGTTC17200.0043185045.4093781
CAAAAGA28756.0967905E-65.2277391
CATCAAA23953.0105657E-45.0801421
TAAATAT32802.463261E-65.01364563
Produced by FastQC (version 0.11.5)