FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1587637
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATTC11703.5488483E-89.8922281
CTCAGAA11904.6127752E-89.7259731
CCCAAAG11402.5748886E-79.5180171
CTCATGT8054.809232E-48.9859521
CTCACCT7450.00238321448.7386881
CTTTAAC16601.3496901E-98.7152921
CTCAAAG17855.875336E-108.5102261
CCCTCTT11202.0210675E-58.3962511
CTCCTTT31550.08.2539761
CCTACAT7900.00405068378.2409151
CTCTTGT12307.3273513E-68.2334711
CTTCCGT14256.3294083E-67.61441331
CCCATCT12457.575344E-57.55325371
CCCAATC9600.00274628547.5350961
CCCGTTT11602.7638834E-47.483131
CCCATAT15703.1325726E-67.3719171
CTTATTT29500.07.3562981
CACAAGA14054.303077E-57.207951
CTATATT20255.435504E-87.14438771
GTTACAT13251.6353368E-47.09720751
Produced by FastQC (version 0.11.5)