FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065371
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT5759.6933945E-915.1715899
CACACGT6251.7953425E-915.0315447
ACACGTC6503.1250238E-914.4534078
GCACACG7753.673631E-812.1222136
AGTCGAG4902.2926106E-410.934894145
AGAGCAC8159.168816E-710.7053893
AGCACAC8802.1353117E-710.6758125
GAGCACA8209.919713E-710.6401124
GAAGAGC12401.6897469E-47.07773161
AAGAGCA15507.8482535E-66.9305572
CAAGTCG6702.1484811E-76.626844140-144
GATCTTT11900.00644281556.20619732
GTTCCGG17150.0082699415.1174271
AAGTCGA7402.8472114E-45.099983140-144
CCGTGTG5100.00464723355.0038433125-129
Produced by FastQC (version 0.11.5)