FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740117
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACACG2309.010871E-418.5670534
CTTGCTT6550.00850896358.7940191
CCTTATT7450.00248266138.6981111
TCAAACT9500.0029006127.4919693
CTTAAAA10750.0086189366.697771
ATCAAAA19952.4888795E-66.4963331
CTTTCAT13850.00218769336.2383561
CTTTTAT15200.00617109335.6842921
CTTCTTT23303.0666255E-55.56231071
Produced by FastQC (version 0.11.5)