FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1137248
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAA16258.529241E-98.4552551
TCACTGT8350.0070295917.75018263
GTCAAAG13151.4940489E-47.14897971
CTCCAGA11350.00180317237.0084711
AATACAG15452.1511936E-56.98009547
CACAAAA18053.4125642E-66.81080061
GTTTGAA16103.5254125E-56.73739241
CTTTCAC14856.594586E-46.3305781
CTGATAG12700.00585705556.26347641
CTTTCAG23508.1171856E-76.1544411
CTTCAAG19900.00377374075.0874651
CTTTTCT27406.860128E-55.01452161
Produced by FastQC (version 0.11.5)