FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2610996
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTC8400.0072045327.7296857
CCCCAAT10900.009335796.64388371
CTCAAAT22201.967339E-66.1979651
CTCAGAA19457.241598E-55.95729161
CAAGAGG14550.0037271955.94977059
CTTGGAT19759.0366455E-55.86680171
TTTGGTA25957.986655E-75.83945132
CTCTTAT21404.641988E-55.75285771
CTCCAAT20601.6573962E-45.62472441
CCCTGAT24551.083293E-55.6046771
CTTTTGT46601.8189894E-125.5945491
CTTTTAT50650.05.43316171
CTCCTGA24556.453299E-55.3096941
CCCAAAA25209.744156E-55.1727381
GTACAAG18400.0083013535.11651231
CATCGCT22756.7897415E-45.09315731
CTCCGAT24303.1407416E-45.0663031