FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences939615
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTCCC4051.4812293E-414.2806131
CTTTAAG8653.1286618E-1013.3725991
CTCCCGT5301.0394267E-412.2766131
CTCAATC6655.3643016E-611.9586731
CTCAAAT8104.0729683E-610.710461
CTTTAAC8901.0926742E-610.5600331
CCCATAC4800.00851252710.543111
TACGATT6204.5175242E-410.4794442
ACGATTC5750.002847335310.0440663
CATAGAG6800.00104182789.5685361
CTTTCTA7853.7572466E-49.2096321
CTCAAGA7954.2646102E-49.0937871
CCAAGAT7954.2646102E-49.0937871
CTACGAT6550.00823271658.8299981
CTCCGGT9051.5968956E-48.7873121
CCCACAT6650.0093198698.6972161
CTTAATT7600.0028676498.5613221
CACCAAA9502.7003943E-48.3710711
CCATAAT11302.2724786E-58.3171941
CCTTCGT8750.00110725948.2623561
Produced by FastQC (version 0.11.5)