FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2073171
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTCGA6455.9369527E-410.1744211
CTCACAT7350.00196105878.9285741
GTTCAAT20801.8189894E-128.7640251
CTTAGAT8300.00588056177.90662861
CCTATCT11250.00150567147.1296311
CTCCCAT13852.5547596E-46.84416531
AGATTGG14909.8397366E-56.7684196
CTTTAAT34650.06.73400831
CCTCATT14153.3198553E-46.69905951
CTCAGAA12150.00338708246.601511
CCCATGT13400.00131072296.52985241
CCCAAAA20402.9319035E-66.43382551
CAGATCT12400.0047588386.39022873
CTCTTGT15409.268406E-46.15530441
CCTTAAT13350.0090246256.00811621
CTCAAAT17255.596232E-45.91787621
CTTCAGA17406.2518363E-45.866861
CTTTAAC20301.21584366E-45.7471281
CCTCAAT15350.0059961065.7003271
TGCGTCA15200.00616342765.6861759
Produced by FastQC (version 0.11.5)