FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937136
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA10404.4201443E-1011.8355921
TCCGATC8807.662675E-811.4970043
CTCTATT5850.00320482239.9016721
TCTATTG6250.00562310179.2496662
CCGATCT8751.1123106E-49.0844954
CTATCAT9452.5090744E-48.4282091
GAGTTTT10004.713643E-47.9489324
TTCCGAT15502.0937017E-56.9931962
ATTTACA13200.00122219156.56936556
CTTTTCG15550.0074649145.58761
CTTTTAT19700.00327320445.1456021
AAAATCC21650.00198757075.00694273
Produced by FastQC (version 0.11.5)