FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences958374
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGAA5450.001667742210.7117851
CTTGTGG6400.00630543159.1217551
AATGCTA6400.00686396249.0285
CTTTAAC7450.00219920358.8156561
ATACCCG8100.00514132588.024898
TCTTCTA8250.0060595537.87939364
CCCTGAA8400.00649493757.8186471
CTTTATT15551.9097479E-57.03929661
CTGAAAT12656.7420193E-46.92243861
CTTCAAT13050.00706578656.1510681
CTCGCCG19556.959788E-55.97231
TTGCCAA13350.0099459445.95104178
ATTGAAA20050.00369859775.0954441
Produced by FastQC (version 0.11.5)