FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886888
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT3551.0728036E-720.3144787
ACACGTC3401.5933329E-619.0896348
CACGTCT3202.0175486E-518.0290999
GAGCACA5506.535138E-714.4240934
GCACACG4602.816571E-514.109736
AGAGCAC5408.559371E-613.3556413
AGCACAC5551.6344825E-411.69455055
CCCATAT7953.8017097E-510.0207411
GTTATAA9703.3168055E-48.2128751
GGTGGAT9200.0017852747.8720251
CTTCCAA10800.0085240086.7057991