FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838178
Sequences flagged as poor quality0
Sequence length100-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC3000.004829271714.4414188
ATTAACC3558.9575024E-414.2380196
GCACACG3250.00820627813.3305416
CCACTGG4800.002052378612.7550955145
CTCCGGA9502.410423E-69.9277581
CCTTCGG6600.0085126388.79381751
CTCATAT10901.2980356E-47.9870461
ACCAGTT8450.007491277.69482762
CGTTTTA8600.0087999327.5574584
CACTGAT9900.00360139027.32818131
CTGAAGA12150.00356506776.5682221
TCAAATT16000.00163463615.86997752
CTGAAAA22301.3454712E-55.85597321
CTTAAAA14950.00472294355.82333141
CTGTTCT18752.7414283E-45.80392031
CTTCCGG17808.887486E-45.7061011
CTTTAAA19150.00223929415.3038431
AATTTAA19250.0025345315.25173855