FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1399668
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAAT12951.8553692E-1010.4514861
CTCAGTT5900.00393685659.6591
CTTTAAA18800.09.4727561
CTCTGAT9603.6477584E-58.9043911
CTCAAGG10551.1543285E-58.7777891
CTTTTAT27400.08.5794131
CTTCTAT15907.570634E-98.5123741
CTCAAAT10951.8450019E-58.4571391
CTCAAAG13755.3417716E-78.2891791
CCCACAG8650.00114470088.2352751
CTATTAT17454.5893103E-98.1644851
CTTTAAC8950.00160306557.9592331
CTCAAAA18109.073119E-97.8712851
CTAAAAG9100.00188819597.82803631
CAACGAT13051.9823934E-57.64208361
CTTCGAC12207.123042E-57.59062861
CTCCGTT12257.49396E-57.5596461
CTCCTAT9450.0027350497.5381091
CTCAATA10509.265352E-47.46272851
CTTTGAT22551.2969394E-97.26566741
Produced by FastQC (version 0.11.5)