FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1635909
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGAAC6200.0052511719.3276429
CTCCAGG10256.584347E-58.467271
AGATTCG8550.00827034757.6110216
CTCCGCT12959.758196E-46.70189331
CTATAAT12150.0036878566.5479061
CTTGCTT15601.698315E-46.49066931
GTTTGAT18453.419854E-56.2720521
CTCTTCT19504.8743337E-45.56343031
CTTTAAA19605.2223547E-45.53504561
TTTGATT32806.157461E-85.51102832
CTTGAAT17550.0048405335.35737751
CAATAAT20559.854793E-45.27916771
AAATCGA18450.0087202615.09464034
Produced by FastQC (version 0.11.5)