FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1655654
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGTT5058.8037935E-411.5636631
CTCACGA6951.00089266E-410.5029661
CTTTTAG10104.9725568E-58.6727471
CCCCGAT9853.599349E-48.1517941
CCCAAAA14457.8377707E-78.082561
CCTTAAC9050.00140622288.0658131
CTCGTAG8200.00522182978.0117141
CTCGTGG9600.00251293797.60370971
CTCTAAA8700.008873527.55127051
CTTCGAA10658.31057E-47.5394531
CTTGAAT14708.64285E-67.44853261
CTCGCCA10050.0039329627.2632451
CTCACGG12355.114496E-47.0926921
CTCACCG15702.179832E-56.97410351
TCACGAA12708.009867E-46.81932162
CTCAAAG18203.3597062E-66.81827161
CTTCTGG16253.524862E-56.7380571
CTTCAAG22956.3688276E-86.67933751
CTTTAAG16454.178683E-56.65613561
CTCCTGT13200.00109164516.6359651
Produced by FastQC (version 0.11.5)