FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977198
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAATT4604.254929E-412.6072781
CCCCAAT4800.00834349410.5717281
GGGCTGA8250.0089734558.737245145
CTTTCGA6650.0091159588.7208241
CCCAGTT7850.0037494288.3111681
CTCTTCT11402.4535779E-58.2666141
CCCAAAA9050.00150434218.0101491
AGTTGAT8200.005552977.95642231
CTTTAAA10054.807409E-47.9344311
CTCCGAT8600.0085074987.58635661
CTCATTT8600.0085074987.58635661
CTCCGTT10050.00420389377.2131191
CTTATAT10250.0050923327.0723761
CTTTGAT15652.2924614E-56.9481011
CTTTAAT10450.0061427846.93701931
AAGAGTT10450.0063604776.91213853
CAAATAC11800.00264637966.7577151
CTCTCCG10800.0084475976.71220831
CTTCCAT16655.430151E-56.53079751
CTTCTTT27357.3250703E-96.36125951
Produced by FastQC (version 0.11.5)