FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726076
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACGA2850.003054055915.4710091
CTCAAAT6654.465188E-612.1557931
CCCATTT7301.2607188E-511.0734281
CTTACCA4650.00595273211.0626041
CCCTCAT4800.007537123710.7168981
CCAAGAT5950.00326009849.8806451
GTTCAAT8759.253501E-59.2384031
CTCCAAA8150.0046461628.1151621
CTTTTGA12707.949375E-57.5223211
CTTCAAA14153.009055E-46.7514831
CTTTATT16503.956449E-56.68066361
CTTTCAT14955.8766676E-46.39019971
CTTTGAT12800.00536903556.3153141
CTTGAAA14150.00221210886.2321381
CTTTATA14150.00221210886.2321381
CTTTTAT16300.00166386275.86095051
CTTTTTG17150.00304680485.57046561
AGATATT18350.008412415.10935544
Produced by FastQC (version 0.11.5)