FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502312
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAT4602.6820046E-514.1806341
CTTAAAG6301.888402E-713.8054851
CTATAGA3200.00721962613.5897741
CCCGTAT3250.00799917813.38071
CTACAAA5509.806816E-613.1779621
CTTCCTT9251.4612488E-710.9697641
AATCTGC7650.00302865968.5082365
CTTCCAT9400.00218497457.71051031
ATCAAAT9500.00242416377.6293471
TTATGTT8600.0087060827.5653599
AGCTTTT12104.4829387E-47.17223174
ATTTAAT10200.00485462977.1057641
CAATAAA12255.0342444E-47.09996271
ATTTTCC10950.00985259756.60456666
TTTGATA12800.00633267976.21500062
TTTTGCT12800.00634579646.2137637
CTTCTTC17450.00439458575.3995661
CTTTTTT22455.6990166E-45.154214
Produced by FastQC (version 0.11.5)