FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1285884
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCAC605.120195E-447.9944763
CTCGGGA4350.004095428611.6328221
ATTGCGC8609.732991E-48.371138
TCAGAGA9753.729192E-48.124672
CTCGGCT10000.00411708657.2289681
CTAAAAA14153.6850286E-46.64145471
CTTTGGA16700.00270637365.62734031
CTTCAAT19050.00219651755.31262731
CTTTCAA23151.5609634E-45.308531
CTTGAAA22204.9216696E-45.21006731