FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4650840
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACTA12105.6654244E-89.598811
CCCACGT12509.2902155E-78.710921
CCCCTCT15502.8953946E-77.9615941
CCCAACT20509.968062E-107.7902541
CTCATGT22409.276846E-117.77760741
CCCAAAC25202.0008883E-117.4895481
CTCACAT17502.5008376E-77.4665041
CTCCAAT40300.07.20506241
CTCAGCT21801.9653453E-76.6597251
CTTTAAT86150.06.65663341
CTTAAAG47700.06.54384331
CTCAGGT19007.183544E-66.4949851
CCCAATT24654.0141458E-86.47871071
CTTAAAT54850.06.352541
CCCCTAT16302.863948E-46.23481041
CTTAAAA74100.06.1717051
CCCACAC20101.7157938E-56.1395381
CCCACGA19205.8034668E-56.049251
CTCACAA23153.8631515E-65.9577931
CTTCTAT72200.05.9319521
Produced by FastQC (version 0.11.5)