FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509557
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGATC2550.001633597116.9892924
CTCAGGA3203.9910246E-415.836741
CTCAACT3000.004742574414.4793041
CTCCAAG4001.3151651E-414.4793031
ACAATCC3700.001223804713.660313
CACGGAT4302.485034E-413.4333933
CCCAAAG4402.951582E-413.1630041
CCTCGAT6353.1447235E-612.541131
CTCAAAG5851.8717108E-512.3754741
CTCCATT5351.1180198E-412.1788541
CTCATAG4250.003399226611.9241331
CTCTCTG4400.00440507111.5176291
CTTTAAT6204.393499E-410.5091721
CTTTCCA8054.36877E-59.89269351
CCTTGAT7903.940327E-49.1641171
CTTTATT9050.00152090287.99961571
CTTCAAA9150.00169493647.9121881
CTCCTTT10256.00774E-47.76938341
CTTTTAT9900.00367013787.31278041
CTTTCTT12556.7246443E-46.92237661
Produced by FastQC (version 0.11.5)