FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2202825
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT5409.546835E-613.2170627
CACGTCT6951.013258E-511.2963089
ACACGTC8508.457197E-610.0760658
GAGCACA14558.134691E-56.8675774
CTCACGG15600.0013420245.9686771
CTCCGCT21503.5692987E-45.3301671
CCTTTAT25201.15825154E-45.11600881
Produced by FastQC (version 0.11.5)