FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382908
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC7903.6808156E-510.0512556
CTTTAAA19654.4019544E-108.1068441
CCGATCT9150.00175074937.8883567
TCAAGCC10550.00699984566.844533
CTCCATT15701.816814E-46.4568411
GATCCTG12700.0059685666.25235655
GTTTGAT17401.0150613E-46.2421431
CTTCCGA14350.00317221026.0358297
AAATGAC13250.0092601485.99282465
CTTCCAA19457.2434E-55.9565131
CTTCATT24902.228724E-65.8159731
CTTCGAT15000.0050142375.7927091
ACGATTT16550.0024639525.67206863
GCTCTTC15400.00671072545.6422491
CTTTTAC22459.597027E-55.48307661
GTTTCAA18700.00170408135.42098471
CTCAAAA17600.0049354035.34838151
CTTCAAT23351.732947E-45.2717371
TTGATTA19450.00289022885.197623
CTTCCAT26553.9978222E-55.1818021