FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468313
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTCTT3305.156376E-415.3115571
TTCACGA3457.3964417E-414.6004847
CCTATAT3558.9696085E-414.2332781
CTTGCCT4000.002205588412.6320351
CTTTTGT8353.7374775E-812.1025481
CATTAAT4450.004898248211.3546371
CTCAAAG5350.001562523210.7937291
CACAAAT4800.00860055210.5266951
CTTTGAT8406.3851094E-610.3118661
CTTTCAG7100.00156442479.1499641
CTTCTTT13704.2922693E-88.9570221
CTTTTTT19856.3191E-45.4546391
Produced by FastQC (version 0.11.5)