FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901354
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTAGA1750.003755028820.6213386
GATTCAC5651.3538913E-512.7742815
CTCATAT7601.7179336E-611.5090061
CTCAACT4500.004956485211.3385011
CTTCGAT9953.7048994E-710.2559311
CTATAAT11251.896442E-79.7187161
CCCTGTT6350.0059671659.1830391
CTCAAAT8054.451724E-49.0547041
CCCAGCG7350.00196524738.9253511
CTTCAAG15653.763489E-98.849311
CCCATCT6600.0081924778.8351961
CTTTAAG7750.0031775138.4646871
CTTTCGC7800.00336769678.4104261
CCCATTT8800.00108011138.2829961
CTCATTT9050.00142482778.0541841
TTCCTGG9900.0037885637.29037246
CTTTGAT15501.8545063E-57.05390641
TTCAAGG11850.00289645986.69975952
CTCAAAA11000.009561446.6263971
CTTCATT17207.8867284E-56.3567181