FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502186
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2253.1976015E-828.6181837
CACGTCT2354.1030726E-521.311419
ACACGTC2951.070396E-519.4021578
GAGCACA3406.9190335E-414.7299474
AATCTTG3951.3067186E-414.4902195
GCACACG4051.6161198E-414.1324356
AGAGCAC3850.001769208913.0082653
AGCACAC3900.001949534812.8414925
GGTGATT4600.006440276310.9451121
ATCTTGT6600.0095294788.6721766
GAATATG10450.0069625636.84645467
Produced by FastQC (version 0.11.5)