FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060239
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT4050.002328298312.5436961
CTTAAAT7301.1147222E-611.9300131
ACGCAGT7450.00241136888.7268386
GTAAATT8200.0057164047.9308962
CCCGCAA9650.0027965147.5206471
CTCAATC9650.0029181957.48800752
CGCGAGC8700.009732547.4729826
CACAGAA10300.0052811477.04604341
AATGCAA12550.00523201056.33168945
TAAATTT14150.00268434696.12626083
TCAATCT14350.00314077856.04087733
CAAGATT13250.00875671956.0250321
AAAGCAA19350.0026940235.22658165
CATTTTC19500.00296723825.1863775
Produced by FastQC (version 0.11.5)