FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences632680
Sequences flagged as poor quality0
Sequence length100-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2457.991356E-519.5445467
ACACGTC3253.6254714E-516.8383798
CACGTCT3450.001085734313.879469
GAGCACA5050.001510032610.839154
AATTCTT6150.00772291388.898335
AAGAGCA8250.0098099767.46541362
GAAGAGC10300.00109131167.3472951
ATAGAGA4050.00144376765.9324317135-139
CGATAGA4050.00933613355.476091135-139
Produced by FastQC (version 0.11.5)