FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499986
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTACC2800.009538121517.544191145
CGGTATA3950.003876482114.923666145
CCTGTAT5407.0166243E-714.3303661
CTGTTAT5500.002436838610.2325921
CTTCTGT6458.2294404E-49.8161491
CTTTAAT6050.00535881339.3023561
CTTTCAA8157.1585114E-48.6317871
AAAGATT10601.5388105E-58.5767972
CAAAGAT7450.0030599348.4985451
CTTTTCT9800.00439662257.1784761
AAGAAAT16507.841029E-56.35762552
CTTTTTT19004.9597677E-45.55387351
Produced by FastQC (version 0.11.5)