FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1987453
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTAG5400.001617597510.7521661
GATCCAC7100.00156160479.1547845
CCTTGGA10250.0050399827.0806951
ACGCTAT10450.0063765636.9111083
CTTTGAT29301.0186341E-106.6879941
GTTTGAG15251.2082407E-46.6628181
GTTACTT15251.2082407E-46.6628181
CTCGAAG14404.349924E-46.5521011
CTAATTT16052.3483542E-46.3307141
GTTTCGA14956.8543025E-46.31105471
CTCCTCT12850.0063784046.2128271
CTATAAG14200.0026534076.1332781
GGTAGAA16603.6282782E-46.1209621
GTTCCGA15100.0052637965.76771831
GTATAAT22006.829684E-55.60823251
CTTCGAT19906.116245E-45.47063731
CTTCAGG25602.1032389E-55.3865831
CAACGAT19550.00289441255.19733861
CATCGAG18500.0091245115.07499465
GTTTTAA21500.00171686285.06352041
Produced by FastQC (version 0.11.5)