FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1219192
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATT9401.9235438E-59.4007381
CTCGAAC6500.0077537258.8952092
CTCCTAC6750.0090607498.7275981
CTTCGAT13151.5613332E-68.3998991
CACAGAT12507.154831E-68.2475811
CTCCAAC8050.00408468168.23294451
CTCATCT11903.4527307E-58.0446091
CTTGCTT10607.1924616E-47.64179471
CAACCAT11702.4825067E-47.55272961
CTTAGAA9850.00296486477.4760521
CTTTCGT10859.223132E-47.46571731
CTTCAAA22856.6302164E-97.08998061
CTTTAAT22052.505476E-87.0132491
CTTCTCT16303.2523585E-56.77660561
CTTAAAT12050.00279819786.72224241
GTTTAAA14200.00225238226.2230241
CTTATGA13050.00643437776.20712851
CTTTGTT17901.1880604E-46.1708761
CTCATAA14400.00263494326.13659241
CATCAAT20451.7888166E-56.1215891
Produced by FastQC (version 0.11.5)