FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597796
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCAAT5802.0629252E-411.4039741
CTGACGA4850.0081326410.6071321
CAAAGGT8355.901047E-48.801471
CTTCAGC7800.00312311768.4798771
CTTCCAT10901.11529385E-48.0908931
CAAAAGA14650.00325861716.0198451
GGTAAGG3650.006949335.195077125-129
AGAAGAA20250.00382342145.08094741